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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
23/04/2019 |
Data da última atualização: |
06/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. |
Afiliação: |
SILVIA DE OLIVEIRA DORTA, ESALQ; JULIANA BALBINOTTE, Centro de Citricultura Sylvio Moreira; ROSE MONNERAT, ESALQ; JOAO ROBERTO SPOTTI LOPES, Centro de Citricultura Sylvio Moreira; TATIANE DA CUNHA, ESALQ; ODIMAR ZANUZO ZANARDI, Fundecitrus; MARCELO PEDREIRA DE MIRANDA, Fundecitrus; MARCOS ANTONIO MACHADO, Centro de Citricultura Sylvio Moreira; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Insect Science, p.1-12, 2019. |
ISSN: |
1744-7917 |
Idioma: |
Inglês |
Conteúdo: |
Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus. MenosBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for... Mostrar Tudo |
Thesagro: |
Doença de Planta; Fruta Cítrica. |
Thesaurus Nal: |
Citrus; Diaphorina citri; Plant diseases and disorders. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196193/1/Dorta-et-al-2019-Insect-Science.pdf
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Marc: |
LEADER 02385naa a2200289 a 4500 001 2108421 005 2019-12-06 008 2019 bl uuuu u00u1 u #d 022 $a1744-7917 100 1 $aDORTA, S. de O. 245 $aSelection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera$bLiviidae) nymphs.$h[electronic resource] 260 $c2019 520 $aBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus. 650 $aCitrus 650 $aDiaphorina citri 650 $aPlant diseases and disorders 650 $aDoença de Planta 650 $aFruta Cítrica 700 1 $aBALBINOTTE, J. 700 1 $aMONNERAT, R. 700 1 $aLOPES, J. R. S. 700 1 $aCUNHA, T. da 700 1 $aZANARDI, O. Z. 700 1 $aMIRANDA, M. P. de 700 1 $aMACHADO, M. A. 700 1 $aASTUA, J. de F. 773 $tInsect Science, p.1-12, 2019.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
17/01/2013 |
Data da última atualização: |
18/09/2015 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
OLIVEIRA, A. C. V. de; MORAES, A. de; RIBEIRO, M. F. S.; PORFIRIO-DA-SILVA, V.; LANG, C. R.; NOGUEIRA, R. R.; OLIVEIRA, E. B. de; MOLETTA, J. L. |
Afiliação: |
A. C. V. de Oliveira, UFPR; A. de Moraes, UFPR; M. F. S. Ribeiro, IAPAR; VANDERLEY PORFIRIO DA SILVA, CNPF; C. R. Lang, IAPAR; R. R. Nogueira, IAPAR; EDILSON BATISTA DE OLIVEIRA, CNPF; J. L. Moletta, IAPAR. |
Título: |
The chemical composition of sunflower seed for animal feed in agroforestry system in Brazil's subtropical. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 2., 2012, Porto Alegre. Invited papers; Offered papers. [S.l.]: INRA, 2012. |
Páginas: |
3 p. |
Idioma: |
Inglês |
Palavras-Chave: |
Integrated systems; Sistema agroflorestal. |
Thesagro: |
Girassol; Helianthus Annuus; Semente. |
Thesaurus NAL: |
crude protein; Eucalyptus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/74415/1/2012-V.Porfirio-ISICS-TheChemical.pdf
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Marc: |
LEADER 00925nam a2200277 a 4500 001 1945487 005 2015-09-18 008 2012 bl uuuu u00u1 u #d 100 1 $aOLIVEIRA, A. C. V. de 245 $aThe chemical composition of sunflower seed for animal feed in agroforestry system in Brazil's subtropical.$h[electronic resource] 260 $aIn: INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 2., 2012, Porto Alegre. Invited papers; Offered papers. [S.l.]: INRA$c2012 300 $a3 p. 650 $acrude protein 650 $aEucalyptus 650 $aGirassol 650 $aHelianthus Annuus 650 $aSemente 653 $aIntegrated systems 653 $aSistema agroflorestal 700 1 $aMORAES, A. de 700 1 $aRIBEIRO, M. F. S. 700 1 $aPORFIRIO-DA-SILVA, V. 700 1 $aLANG, C. R. 700 1 $aNOGUEIRA, R. R. 700 1 $aOLIVEIRA, E. B. de 700 1 $aMOLETTA, J. L.
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Embrapa Florestas (CNPF) |
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